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KMID : 0613820090190111672
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Journal of Life Science
2009 Volume.19 No. 11 p.1672 ~ p.1678
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Toxicity and Characteristics of Antifungal Substances Produced by Bacillus amyloliquefaciens IUB158-03
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Kim Hye-Young
Lee Tae-Soo
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Abstract
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The purified antifungal substances produced by Bacillus amyloliquefaciens IUB158-03 was positive to ninhydrin but negative to aniline, suggesting that the antifungal substance could be a peptide. FAB-MS, UV adsorption spectrum, and amino acid composition analysis revealed that the molecular weight of the antifungal substance was 1042 and that maximal adsorption was at 220 ㎚ and 277 ㎚. The antifungal substance was composed of Asn₃, Gln₂, Ser₁, Gly₁, and Tyr₁. The composition and structural characteristics of antifungal substance were analysed by ¹H-NMR spectrum, ¹H-COSY, HMQC, which revealed that the compound belongs to the iturin A family. Temperature and pH had little effect on the stability of the antifungal substance in the ranges of -70～121℃ and pH 6.0～10.0, respectively. It showed strong antibiotic activity against fungi. An in vitro cytotoxicity test using NIH3T3 cell showed that the antifungal substance does not have cytotoxicity. The number of circulating leukocytes and the hematobiological analysis of the mice administered with the antifungal substances was similar to those of the control group, indicating no cytotoxicity in vivo. Therefore, the antifungal substances extracted from culture broth of Bacillus amyloliquefaciens IUB158-03 have future potential as biocontrol agents against plant diseases caused by fungi.
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KEYWORD
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Antifungal substance, Bacillus amyloliquefaciens, cytotoxicity
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